Blood mononuclear cell nitric oxide production and plasma cytokine levels in healthy gabonese children with prior mild or severe malaria.

Healthy Gabonese Children with Prior Mild Production and Plasma Cytokine Levels in Blood Mononuclear Cell Nitric Oxide

Inverse relationship of plasma prostaglandin E2 and blood mononuclear cell cyclooxygenase-2 with disease severity in children with Plasmodium falciparum malaria.

Prostaglandins (PGs) derived from inducible cyclooxygenase (COX)-2 are important proinflammatory mediators of the host-immune response to infection. Since the role of host-derived PG in human malaria is unknown, plasma bicyclo-PGE2 (a stable catabolite of PGE2), peripheral blood mononuclear cell COX-2 protein, and mRNA were measured in Gabonese children with and without malaria (n=129). Relativ...

Interferon-gamma responses are associated with resistance to reinfection with Plasmodium falciparum in young African children.

The contribution of T cell-mediated responses was studied with regard to resistance to reinfection in groups of Gabonese children participating in a prospective study of severe and mild malaria due to infection with Plasmodium falciparum. In those admitted with mild malaria, but not in those with severe malaria, production of IFN-gamma by peripheral blood mononuclear cells (PBMC) in response to...

Evaluation of Effect of Plasmas Prepared from Packed Red Blood Cells at Different Weeks on Production of Nitric Oxide (NO) and Malondialdehyde (MDA) from Peripheral Blood Mononuclear Cells in Vitro

Introduction: In present study, we evaluated the effect of plasma collected from packed red blood cells (PRBCs) at various weeks after donation on peripheral blood mononuclear cells (PBMCs). Methods: In this experimental study, plasma prepared from twenty PRBCs in the first, second, fourth, and fifth weeks after donation. PBMCs were also isolated from healthy donors and treated with different c...

Differential regulation of beta-chemokines in children with Plasmodium falciparum malaria.

Chemokines regulate the host immune response to a variety of infectious pathogens. Since the role of chemokines in regulating host immunity in children with Plasmodium falciparum malaria has not previously been reported, circulating levels of beta-chemokines (MIP-1alpha, MIP-1beta, and RANTES) and their respective transcriptional profiles in ex vivo peripheral blood mononuclear cells (PBMCs) we...